CD117 (c-Kit) Monoclonal Antibody (ACK2), APC, eBioscience™

来自 : 发布时间：2024-12-25

Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.    Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.03 µg of Rat IgG2b K Isotype Control APC (Product # 17-4031-82) (left) or 0.03 µg of Anti-Mouse CD117 (c-Kit) APC (right). Total viable cells were used for analysis.   Figure 3 Ergosterol Enhances Murine Hematopoietic Cell Homing (A) Structure of ergosterol, a member of the sterol family of compounds. (B) BLI at 1 dpt of fish receiving ergosterol-treated cells in the secondary screen. The region of Interest (ROI) and donor cell radiance values of the WKM are shown. Some residual signal can be seen at the injection site. Right panel shows difference in radiance between vehicle and ergosterol-treated homed donor cells. Data shown are mean +- SD from five individual recipients. (C) Flow cytometry gating for the identification of Lin - SCA-1 + KIT + donor cells after murine HCT. Initial gates were used to identify mononuclear cells that were propidium iodide (PI) negative. (D) Ten million CD45.1 donor cells in 50 muM ergosterol (circulating concentration estimated at 10 muM) or equal v/v vehicle were transplanted into CD45.2 lethally irradiated recipients. Bilateral femur marrow was harvested from recipients 16 hr post-transplant. Flow cytometry was used to quantify absolute numbers of homed donor cells using the gating strategy in (C). Data shown are mean +- SD and p values from a Student\'s t test (n = 10-11 animals/group in two pooled experiments).   Figure 5 miR-17 plays a central role in regulating the pro-B to pre-B transition. ( a ) Outline of experimental strategy. HSCs were enriched from the Mb1tKO donors, transduced with miRNA-expressing lentiviruses and mixed with bone marrow cells from the congenic WT mice, to generate mixed bone marrow chimeras. Reconstituted mice were analysed 8 weeks later. Ratios of lentivirus-transduced cells (GFP + ) versus the WT donor-derived cells (CD45.1 + ) in pro-B cells (B220 + cKit + ) and pre-B cells (B220 + CD25 + ) were quantified. ( b ) Representative flow cytometry plots of pro-B cells and pre-B cells in the bone marrow of recipient mice of indicated groups, showing contributions from WT-derived cells (CD45.1 + ) and lentivirus-transduced cells (GFP + ). A reconstitution group with control virus-transduced CD45.2 + WT HSCs mixed with the CD45.1 + WT BM cells is included for comparison. ( c ) Changes in ratios of GFP + cells versus CD45.1 + cells during the pro-B to pre-B transition. The dash line marks the pre-B/pro-B ratio in Mb1tKO mice. Data are representative ( b ) or pooled ( c ) from 6 independent experiments (mean+-s.e.m. in c ) with n =3 (WT-Vector), 8 (tKO-Vector and 4 x 17), 11 (FL), 7 (del17 and 4 x 19), 4 (del18, del19 and del92) or 6 (4 x 18 and 4 x 92) in b , c .   Figure 6 Ablation of Pten and Phlpp2 do not rescue early B-cell development in mice deficient of the miR-17~92 miRNA family. ( a ) Phospho-Akt T308 (p-AKT) and Bim protein levels were measured in pro-B and pre-B cells of control and Mb1tKO mice by flow cytometry and presented as overlay histograms. ( b ) Representative flow cytometry plots and cell numbers of IgM + B cells (B220 + IgM + ), pro-B cells (B220 int ckit + ) and pre-B cells (B220 int CD25 + ) in the bone marrow of mice of indicated genotypes. ( c ) Representative flow cytometry plots and cell numbers of splenic IgM + B cells (B220 + IgM + ) in mice of indicated genotypes. Data are representative of 2 ( a ) or 8 ( b , c ), or pooled from 8 ( b , c ) independent experiments (mean+-s.e.m. in b , c ) with n =4 ( a : Phospho-Akt(T308)) or 2 ( a : Bim) in a , n =5 (Control, Mb1tKO, Mb1tKO;Pten fl/+ Phlpp2 fl/+ and Mb1tKO;Pten fl/+ Phlpp2 fl/fl ), 7 (Mb1tKO;Pten fl/fl ), 9 (Mb1tKO;Pten fl/+ ), 8 (Mb1tKO;Phlpp2 fl/fl ), 6 (Mb1tKO;Phlpp2 fl/+ and Mb1tKO;Pten fl/fl Phlpp2 fl/+ ) or 3 (Mb1tKO;Pten fl/fl Phlpp2 fl/fl ) in b (BM Ig+ and preB) and c , and n =4 (Control and Mb1tKO), 5 (Mb1tKO;Pten fl/fl , Mb1tKO;Pten fl/+ Phlpp2 fl/+ , Mb1tKO;Pten fl/+ Phlpp2 fl/fl and Mb1tKO;Pten fl/fl Phlpp2 fl/+ ), 9 (Mb1tKO;Pten fl/+ ), 6 (Mb1tKO;Phlpp2 fl/fl and Mb1tKO;Phlpp2 fl/+ ) or 3 (Mb1tKO;Pten fl/fl Phlpp2 fl/fl ) in b (proB). Description: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.Applications Reported: The ACK2 antibody has been reported for use in flow cytometric analysis.Applications Tested: The ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered. c-Kit, also known as mast/stem cell growth factor receptor (SCFR) or CD117, is a trans membrane type III tyrosine kinase receptor encoded by the c-Kit proto-oncogene, located on chromosome 4 in humans. It is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis. 蛋白别名: belly-spot; c-kit proto-oncogene protein; CD117; ckit; dominant spotting; Dominant white spotting; Mast/stem cell growth factor receptor Kit; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit 基因别名: Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; Kit; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W Host server : magellan-srch-3-prod-green:8080/10.253.228.100:8080. git-commit: 2cd8645d2fc6bfe4ccb4abfa14772b0a94f68e98 git-url: http://victoria.invitrogen.com:8333/magellan/core.git git-branch: origin/release/1.27.0-2021.08.32-1.0