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当前位置: 首页 > 产品中心 > Other_reagents > A11008/eBioscience/Alexa Fluor®488山羊抗兔IgG(H+L)抗体
商品详细A11008/eBioscience/Alexa Fluor®488山羊抗兔IgG(H+L)抗体
A11008/eBioscience/Alexa Fluor®488山羊抗兔IgG(H+L)抗体
A11008/eBioscience/Alexa Fluor®488山羊抗兔IgG(H+L)抗体
商品编号: A11008
品牌: 默克
市场价: ¥3126.20
美元价: 1875.72
产地: 美国(厂家直采)
公司:
产品分类: 其他试剂
公司分类: Other_reagents
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

RabbitIgG(H+L)Cross-AdsorbedSecondaryAntibody(A-11008)inIF

ImmunofluorescenceanalysisofGoatanti-RabbitIgG(H+L)Cross-AdsorbedSecondaryAntibodyAlexaFluor®488conjugatewasperformedusingHeLacellsstainedwithalphaTubulinRabbitPolyclonalAntibody(Product#PA5-16891).Thecellswerefixedwith4%paraformaldehydefor10minutes,permeABIlizedwith0.1%Triton™X-100for10minutes,blockedwith1%BSAfor1hourandlabeledwith2µg/mlRabbitprimaryantibodyfor3hoursatroomtemperature.Goatanti-RabbitIgG(H+L)Cross-AdsorbedSecondaryAntibodyAlexaFluor®488conjugate(Product#A-11008)wasusedataconcentrationof4µg/mlinphosphatebufferedsalinecontaining0.2%BSAfor45minutesatroomtemperature,fordetectionofalphaTubulininthecytoplasm(Panela:green).Nuclei(Panelb:blue)werestainedwithDAPIinSlowFade®GoldAntifadeMountant(Product#S36938).F-actinwasstainedwithRhodaminePhalloidin(Product#R415,1:300)(Panelc:red).Paneldrepresentsthecompositeimage.Nononspecificstainingwasobservedwiththesecondaryantibodyalone(panelf),orwithanisotypecontrol(panele).Theimageswerecapturedat60Xmagnification.

ProductDetails

TESTEDAPPLICATIONSDILUTION
FlowCytometry(Flow)1-10µg/mL
Immunocytochemistry(ICC)4µg/mL
Immunofluorescence(IF)4µg/mL
PUBLISHEDAPPLICATIONS
Immunohistochemistry(Frozen)(IHC(F))See20publicationsbelow
Immunohistochemistry(IHC)See20publicationsbelow
Immunocytochemistry(ICC)See26publicationsbelow
Immunohistochemistry(Paraffin)(IHC(P))See8publicationsbelow
FlowCytometry(Flow)See2publicationsbelow
WesternBlot(WB)See3publicationsbelow
Immunohistochemistry-FreeFloating(IHC(Free))See1publicationsbelow
ChIPassay(ChIP)See1publicationsbelow
MiscellaneousPubMed(MISC)See472publicationsbelow
SpeciesreactivityRabbit
Host/IsotypeGoat/IgG
ClassPolyclonal
TypeSecondaryAntibody
ImmunogenGammaImmunoglobinsHeavyandLightchains
ConjugateAlexaFluor®488
Formliquid
Concentration2mg/ml
Purificationpurified
StoragebufferPBS,pH7.5
Contains5mMsodiumazide
Storageconditions4°C,storeindark
RRIDAB_143165
TargetIgG
CrossAdsorptionAgainsthumanIgG,humanserum,mouseIgG,mouseserumandbovineserum
AntibodyFormWholeAntibody

ProductSpecificInformation

AlexaFluordyesareamongthemosttrustedfluorescentdyesavailabletoday.Invitrogen™AlexaFluor™488dyeisabright,green-fluorescentdyewithexcitationideallysuitedtothe488nmlaserline.Forstablesignalgenerationinimagingandflowcytometry,AlexaFluor488dyeispH-insensitiveoverawidemolarrange.Probeswithhighfluorescencequantumyieldandhighphotostabilityallowdetectionoflow-abundanceBIOLOGicalstructureswithgreatsensitivity.AlexaFluor488dyemoleculescanbeattachedtoproteinsathighmolarratioswithoutsignificantself-quenching,enablingbrighterconjugatesandmoresensitivedetection.Thedegreeoflabelingforeachconjugateistypically2-8fluorophoremoleculesperIgGmolecule;theexactdegreeoflabelingisindicatedonthecertificateofanalysisforeachproductlot.

Thegoatanti-rabbitIgGwholeantibodyconjugatesaremostcommonlypreparedbyimmunizingthehostanimalwithapooledpopulationofimmunoglobulinsfromthetargetspeciesandcanbefurtherpurifiedandmodified(e.g.,immunoaffinitychromatography,antibodyfragmentation,labelconjugation,etc.)togeneratehighlyspecificreagents.Inthefirstroundofpurification,wholeimmunoglobulinsbindingtotheimmunizingantibodyarerecoveredandmainlyconsistofthe~150-kDaIgGclass.FurtherpurificationwithProteinAorGremovesallimmunoglobulinclassesexceptIgGsuchthattheaffinity-purifiedantibodiesreactwithIgGheavychainsandallclassesofimmunoglobulinlightchainsfromrabbit.Tominimizecross-reactivity,thesegoatanti-rabbitwholeantibodieshavebeencross-adsorbedagainsthumanIgG,humanserum,mouseIgG,mouseserum,andbovineserum.Cross-adsorptionorpre-adsorptionisapurificationsteptoincreasespecificityoftheantibodyresultinginhighersensitivityandlessbackgroundstaining.Thesecondaryantibodysolutionispassedthroughacolumnmatrixcontainingimmobilizedserumproteinsfrompotentiallycross-reactivespecies.Onlythenonspecific-bindingsecondaryantibodiesarecapturedinthecolumn,andthehighlyspecificsecondariesflowthrough.Thebenefitsofthisextrastepareapparentinmultiplexing/multicolor-stainingexperimentswherethereispotentialcross-reactivitywithotherprimaryantibodiesorinimmunohistochemistryexperimentswheretherearemaybethepresenceofendogenousimmunoglobulins.Forahighlycross-adsorbedsecondaryantibodyequivalent(orequivalentsecondaryantibodypreparation),pleaseseeproductcatalognumber:A11034.

Usingconjugatesolutions:Centrifugetheproteinconjugatesolutionbrieflyinamicrocentrifugebeforeuse;addonlythesupernatanttotheexperiment.Thisstepwilleliminateanyproteinaggregatesthatmayhaveformedduringstorage,therebyreducingnonspecificbackgroundstaining.Becausestainingprotocolsvarywithapplication,theappropriatedilutionofantibodyshouldbedeterminedempirically.Forthefluorophore-labeledantibodiesafinalconcentrationof1-10µg/mLshouldbesatisfactoryformostimmunohistochemistryandflowcytometryapplications.

Background/TargetInformation

WeofferanextensivelineofInvitrogen™secondaryantibodyconjugateswithwell-characterizedspecificityandlabeledwithawideselectionofpremiumfluorescentdyes,includingInvitrogen™AlexaFluor™fluorescentdyes.Fluorescentsecondaryantibodyconjugatesareusefulinthedetection,sorting,orpurificationofitsspecifiedtargetandidealforfluorescencemicroscopyandconfocallaserscanningmicroscopy,flowcytometry,andfluorescentwesterndetection.ThebreadthoffluorescentMarkersweofferallowsourreagentstobetailoredtoalmostanyfluorescentdetectionsystem.

Secondaryantibodiesmaybeprovidedinthreeformats:wholeIgG,divalentF(ab')2fragments,andmonovalentFabfragments.Becauseofthehighdegreeofconservationinthestructureofmanyimmunoglobulindomains,mostclass-specificsecondaryantibodiesmustbeaffinity-purifiedandcross-adsorbedtoachieveminimalcross-reactionwithotherimmunoglobulins.

ForResearchUseOnly.Notforuseindiagnosticprocedures.Notforresalewithoutexpressauthorization.


品牌介绍
eBioscience公司创建于1999年,位于美国加利福尼亚州圣地亚哥,在供应免疫学和肿瘤学流式细胞仪试剂方面居世界领先地位。2011年底,被生物芯片产业的先驱,全球50家最具创新性公司之一的Affymetrix(昂飞)公司收购,成为其生物学全面战略的重要组成部分。eBioscience公司是多色流式检测试剂供应业的领导者。在免疫学、肿瘤学、细胞生物学、干细胞生物学和诊断学领域,eBioscience为客户提供新型的荧光标记抗体、荧光染料和免疫学相关试剂。eBioscience公司往往率先将重要的试剂推进市场,现在每年推出800多种多色流式新产品。